P12 Saur, Schnieke
Temporal and spatial analysis of the microbiome in a porcine model for colorectal cancer
Principal Investigators:
Saur, Dieter, TUM
Schnieke, Angelika, TUM
An existing APC1311 porcine model of colorectal cancer will be used to study causal relationships between changes in gut microbial composition and cancer progression and regression. We will experimentally manipulate the mutational load in individual polyps by in vivo and ex vivo CRISPR/Cas9 gene editing and monitor changes in microbiome composition and in lipid and bile acid metabolism during disease progression. We will also generate a new model of inflammatory bowel disease based on the TnfDARE mouse by modifying the porcine TNF gene.
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